Case study
A structure from about a milligram – impure, and no other option.
A pharmaceutical company sent us about 1 mg of an impure HPLC fraction – far too little, and too impure, for any conventional method. We crystallised it directly on the grid and solved the structure by electron diffraction.
The challenge
About 1 mg, and not an option for anything else.
A leading pharmaceutical company approached us with an HPLC fraction containing about 1 mg of an unknown natural product – a honey-like, gel-type substance, clearly not fully pure and difficult to analyse by any other method. The task: obtain structural information from it. Conventional approaches were simply not an option.
What ELDICO did
Precision from a scarce, impure sample.
Drawing on our expertise in micro-filtration and micro-crystallisation, we worked the material down to a single measurable crystallite – and solved it.
Under 100 µg crystallised on-grid
From the ~1 mg submitted, we crystallised under 100 µg directly onto a 3 mm TEM grid, ready for measurement.
New compound solved
Determined the structure of a new macro-cyclic natural compound by electron diffraction.
Insight from an impure sample
A definitive structure from about a milligram of impure material – no bulk crystallisation required.
Download the case study if
This sounds like your sample.
- You don't have enough material available.
- You haven't been able to isolate your compound of interest.
- You haven't been able to crystallise your target molecule.
- You haven't been able to structurally characterise your (new) compound.
Download the full case study.
See how, from about 1 mg submitted, we crystallised under 100 µg on the grid and solved a new macro-cyclic natural product by electron diffraction.
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